Wells B446 Bedienungsanleitung PDF herunterladen (Seite 5)

Pharmacology+&+Toxicology+Core+Facilities+ + SOP+#:15928.B446.001++

+ + Version+#:++

Author:+J.+Thompson+&+T.++Szasz+ Page%%%%%%of%%8+ Approval+Date:+

+ + +

f. Click%on%OK%and%the%software%will%return%to%the%initial%window.%

g. Click% on% Read% Plate.% A% window% will% open% requesting% “Results%

ID”.%Type%in%the%desired%name%for%the%reading%results.%%

h. Click% on% the% “Read% Plate…”% button.% A% window% will% open%

requesting%“Plate%ID”.%Type%in%the%desired%name%for%the%plate.%%

i. Open%the%lid%of%the%µQuant%Spectrophotometer.%%

j. Remove%lid%of%the%96‐well% plate.%Insert%plate%with%the%A1%well%in%

the%upper%left%corner.%%

k. Close%the%lid%of%the%µQuant%Spectrophotometer.%

l. Click% on% “Read% Plate”.% The% spectrophotometer% will% begin% the%

absorbance%measurement%of%the%plate.%%

m. When%scan%is%done,%a%window%will%open%displaying%the%results%

(absolute,% blanked% and% calculated% values).% Check% standard%

curve%by%clicking%on%the%“Standard%Curve…”%button,%which%will%

open%another%window.%If%desired,%a%copy%of%the%standard%curve%

can% be% printed% from% this% window.% Click% OK% to% close% the%

standard%curve%window.%

n. Under% the% “Results”% menu,% select% “Print% results…”% to% obtain% a%

copy%of%the%scan%results.%

o. To% close% the% results% window% and% exit% the% KC% Junior% software,%

click%on%the%“X”%button%on%the%upper%right%corner.%%

p. A% window% will% open% requesting% whether% results% should% be%

saved.%Make%the%appropriate%selection%for%saving%on%a%USB%flash%

drive%or%select%“no”%if%results%were%printed.%

q. A% window% will% open% requesting% whether% protocol% should% be%

saved.% Select% “No”% to% keep% protocol% defaults.% For% changes% to%

defaults,%create%New%Protocol%instead,%as%needed.%%

r. Open%lid%of%the%µQuant%Spectrophotometer.%Remove%plate%and%

close%the%lid%of%the%µQuant%Spectrophotometer.%

s. Shut%down%computer%and%µQuant%Spectrophotometer.%

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Wells B446 Bedienungsanleitung Seite 5